Please use this identifier to cite or link to this item: http://dspace.iitrpr.ac.in:8080/xmlui/handle/123456789/1175
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTolchard, J.-
dc.contributor.authorPandey, M.K.-
dc.contributor.authorBerbon, M.-
dc.contributor.authorNoubhan, A.-
dc.contributor.authorSaupe, S.J.-
dc.contributor.authorNishiyama, Y.-
dc.contributor.authorHabenstein, B.-
dc.contributor.authorLoquet, A.-
dc.date.accessioned2019-01-01T07:16:59Z-
dc.date.available2019-01-01T07:16:59Z-
dc.date.issued2019-01-01-
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/1175-
dc.description.abstractWe present a new solid-state NMR proton-detected three-dimensional experiment dedicated to the observation of protein proton side chain resonances in nano-liter volumes. The experiment takes advantage of very fast magic angle spinning and double quantum 13C–13C transfer to establish efficient (H)CCH correlations detected on side chain protons. Our approach is demonstrated on the HET-s prion domain in its functional amyloid fibrillar form, fully protonated, with a sample amount of less than 500 µg using a MAS frequency of 70 kHz. The majority of aliphatic and aromatic side chain protons (70%) are observable, in addition to Hα resonances, in a single experiment providing a complementary approach to the established proton-detected amide-based multidimensional solid-state NMR experiments for the study and resonance assignment of biosolid samples, in particular for aromatic side chain resonances.en_US
dc.language.isoen_USen_US
dc.subjectAmyloid fibrilsen_US
dc.subjectSolid-state NMRen_US
dc.subjectProton detectionen_US
dc.subjectVery fast MASen_US
dc.subjectProtein NMRen_US
dc.titleDetection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMRen_US
dc.typeArticleen_US
Appears in Collections:Year-2018

Files in This Item:
File Description SizeFormat 
Full Text.pdf1.37 MBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.