Nanocrystalline Titanosilicate–Acetylcholinesterase electrochemical biosensor for the Ultra-Trace detection of toxic organophosphate pesticides

Abstract

In this work, nanocrystalline titanosilicate and ZSM-5 were synthesized in the presence of propyltriethoxy silane as an additive. Electrochemical biosensors based on nanocrystalline zeolites were fabricated through the covalent immobilization of the enzyme acetylcholinesterase for the ultra-trace detection of toxic organophosphate pesticides such as malathion, parathion methyl, monocrotopos, and dichlorvos. The results show that, among the zeolite-based biosensors investigated in this study, the nanocrystalline titanosilicate-based acetylcholinesterase biosensor exhibited the highest electrocatalytic activity. The nanocrystalline titanosilicate-based acetylcholinesterase biosensor exhibited excellent sensitivity, selectivity, stability, linear range, and limit of detection. The analytical performance of the developed sensor was demonstrated in the determination of these pesticides in a range of real samples (apples, cabbages, tap water, and river water) with satisfactory recovery