Electrochemical and spectroscopic detection of insulin using AgNPs-decorated organic derivative

Abstract

Insulin is a crucial hormone that plays a vital role in regulating blood glucose levels in the body. The accurate detection of insulin is essential for the diagnosis and management of diabetes, a chronic disease that affects millions of people worldwide. In present work, we present an approach for the detection of insulin using a 4Hchromene derivative surface decorated with AgNPs. The method involves the use of cyclic voltammetry, linear sweep voltammetry, chronoamperometry, UV–visible and fluorescence spectroscopy, all of which are carried out in an aqueous medium. Our results demonstrate that the proposed method exhibits a low detection limit of 2 nM, high sensitivity (1.77 μA nM-1 cm-2), and excellent reproducibility (3.21 %). The electrochemical and spectroscopic measurements provide accurate and reliable detection of insulin in various concentrations. The method’s property of detecting insulin in both liquid and solid state makes it a cost-effective and environment friendly alternative to conventional detection methods, with significant implications in the field of diabetes management.