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Title: | Recognition of bovine hemoglobin protein on molecularly imprinted polymer surfaces using nonlinear vibrational spectroscopy |
Authors: | Chaudhary, S. Kaur, H. Kaur, H. Jena, K.C. |
Issue Date: | 7-Jul-2024 |
Abstract: | Advancement in molecularly imprinted biomimetics has aided in developing robust artificial recognition-based materials, which can be customized for bio/chemo-sensing of distinct molecules. The present study reports a simple one-step synthesis and analysis of protein-imprinted polymer thin films as a recognition element directly onto a solid support. Dopamine has been explored as a versatile functional monomer for a molecularly imprinted polymer (MIP) matrix to fabricate polydopamine (PDA) thin films with bovine hemoglobin as a template protein molecule. A detailed molecular-level insight into the recognition of the template molecule at each step has been investigated using vibrational sum frequency generation (VSFG) spectroscopy. A suitable PDA-coated thin film is selected based on the extent of polymerized intermediates formed after non-imprinted polymer fabrication at different time durations. An optimally prepared film of MIP is specified by observing the spectral signature of the methyl groups from protein molecules at the air–polymer interface. The template removal from MIP films after the washing procedure and subsequent re-binding of the protein molecules were evaluated by VSFG spectroscopy. The insightful molecular-level findings from SFG spectroscopy demonstrate the fabrication of an imprinted matrix as a label-free chemical sensor. |
URI: | http://dspace.iitrpr.ac.in:8080/xmlui/handle/123456789/4672 |
Appears in Collections: | Year-2023 |
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