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UV-FIA: UV-induced fluoro-immunochemical assay for ultra-trace detection of PETN, RDX, and TNT

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dc.contributor.author Chaudhary, S.
dc.contributor.author Sonkusre, P.
dc.contributor.author Chopra, A.
dc.contributor.author Bhasin, K.K.
dc.date.accessioned 2019-08-22T11:09:44Z
dc.date.available 2019-08-22T11:09:44Z
dc.date.issued 2019-08-22
dc.identifier.uri http://localhost:8080/xmlui/handle/123456789/1303
dc.description.abstract Fluorescence quenching based immunoassay format for the detection of a trace amount of some nitroexplosives with a high degree of selectivity is reported in this study. The immunoassay comprises antiexplosive antibodies functionalized microtitre strips specific to the targeted explosives, pentaerythritol tetranitrate (PETN), 1,3,5-trinitroperhydro-1,3,5-triazine (RDX), and 2,4,6-trinitrotoluene (TNT). UV induced photolysis of nitro-explosive bound to targeted antibodies generates primarily nitrite ions which after the quick reaction with the detector molecule, 2,3-diaminonaphthalene (DAN), a fluorophore, quenches its fluorescence intensity, however, proportionately undergo cyclization to produce a highly fluorescent product, 2,3-naphthotriazole (NAT). The synthesized product, NAT, was verified using various chromatographic and spectrophotometric techniques. This newly developed antibody-based detection method, utilizing DAN dye, demonstrated a high selectivity towards PETN, RDX, and TNT. This method can be used as an economical testing kit for direct quantification of explosives, implying the great potential for quick, low-cost trace detection of explosives. en_US
dc.language.iso en_US en_US
dc.subject Charge-transfer fluorophore en_US
dc.subject 2,3-Diaminonaphthalene (DAN) en_US
dc.subject 2,3-Naphthotriazole (NAT) en_US
dc.subject Nitro-explosives en_US
dc.title UV-FIA: UV-induced fluoro-immunochemical assay for ultra-trace detection of PETN, RDX, and TNT en_US
dc.type Article en_US


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