Abstract:
The pronounced demand of sensing of biological analytes which play a significant role in progress of cancer cells
encouraged us to develop a novel chemosensor having high selectivity and sensitivity for NADH. Therefore,
imine linked dipodal receptor (L1) was synthesized and its organic nanoparticles (L1-ONP) were prepared using
reprecipitation method to enhance their water solubility. These ONPs form a metal complex with Co(II) which
was characterized by UV–vis absorption and Fluorescence Spectroscopy. The ONPs show highly sensitive affinity
having a low detection limit (LOD) of 16.95 nM for the cobalt ions. The prepared ONPs metal complex exhibits
high selectivity and sensitivity to sense the NADH with a very low LOD of 83.89 nM without any interference
from other potentially interfering biological molecules. Fluorescence response from NADH was also not affected
by pH and UV irradiation. The sensor was also employed for detection of NADH in MCF-7 cells.
