Abstract:
A rapid fluorescence method has been developed which describes the sensitive and selective
determination of ketoprofen by developing hybrid assemblies composed of silver nanoparticles and organic
nanoparticles. Three different ligands were synthesized using various thiourea derivatives and these were
processed with organic nanoparticles using a single step re-precipitation method. Hybrid nanoassemblies
were developed and consisted of silver nanoparticles and organic nanoparticles, respectively. These were
further analysed for their response to various drug molecules and their fluorescence emission spectra were
recorded. A non-selective response was observed for hybrid nanoassemblies composed of ethyl
isothiocyanate and phenyl isothiocyanate. However, a turn-on fluorescence response to ketoprofen was
noticed for the hybrid nanoassembly composed of 1-naphthyl isothiocyanate. Titrations were carried out
revealing that with subsequent addition of ketoprofen, a gradual increase in fluorescence emission was
noticed. Our sensor can effectively determine ketoprofen up to a detection limit of 34 nM. Moreover, other
drug molecules were found to have negligible interference in the sensing of ketoprofen in aqueous media.
