Abstract:
Head and neck cancer detection using fuorescence spectroscopy from human saliva is reported here. This study has been
conducted on squamous cell carcinoma (SCC), and dysplastic (precancer) and control (normal) groups using an in-house
developed compact set-up. Fluorescence set-up consists of a 375-nm laser diode and optical components. Spectral bands of
favin adenine dinucleotide (FAD), porphyrins, and Raman are observed in the spectral range of 400 to 800 nm. Presence of
FAD and porphyrin bands in human saliva is confrmed by the liquid phantoms of FAD and porphyrin. Signifcant diferences
in fuorescence intensities among all the three groups are observed. Three spectral ranges from 455 to 600, 605 to 770, and
400 to 800 nm are selected for each group and area values under each spectral range are computed. To diferentiate among
the groups, receiver operating characteristic (ROC) analysis is employed on the area values. ROC diferentiates among the
groups with accuracies of 98%, 92.85%, and 81.13% respectively in the spectral ranges of 400 to 800 nm. However, in other
two spectral ranges (455 to 600 and 605 to 770 nm), low accuracy values are found. Obtained accuracy values indicate that
selection of human saliva for head and neck cancer detection may be a good alternative.
