Abstract:
In this work, a nanoporous polyaniline–nanocrystalline zeolite organic–inorganic hybrid material was
synthesized. An acetylcholinesterase electrochemical biosensor based on a polyaniline–zeolite nanocomposite
material was fabricated for the sensitive detection of neurotransmitter acetylcholine and the
resultant biosensor was further employed for the detection of toxic organophosphate pesticides. The
results demonstrate that the polyaniline–nanocrystalline zeolite based acetylcholinesterase biosensor
exhibited much higher activity when compared to conventional polyaniline. The nanoporous polyaniline–
nanocrystalline zeolite based biosensor exhibited good stability, sensitivity, linear range, and limit of detection.
The analytical performance of the developed biosensor was demonstrated in the determination of these
pesticides in different real samples (apple, cabbage, tap water, and river water) with satisfactory recovery
