Abstract:
A non-enzymatic electrochemical biosensor was developed for highly sensitive detection of creatinine
using copper nanoparticles supported over polymelamine formaldehyde. The synergy between the
electrodeposited copper nanoparticles over the highly porous polymer (eCu-PMF) provided a greener
platform to boost up the electron transport at the electrode electrolyte interface by eliminating the role
of redox species as well as interference of major interferents like glucose, dopamine, and ascorbic acid
in physiological media 0.1 M PBS (pH 7.4). The proposed sensor exhibited a wide detection range of
100 fM–60 mM with high sensitivities of 0.320 mA nM 1 cm 2 and 3.8 mA nM 1 cm 2
. Moreover, the
sensor was applied to real samples of serum creatinine and recoveries of 97 to 114% were found. Additionally, a paper-based flexible screen-printed electrode was fabricated which displayed an excellent
activity with the same detection range of 100 fM–60 mM and long-term storage stability of 15 days.
